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Alignment of the amino acid sequences of <t>CTX-M-213</t> (from isolates KA1 to KA6) and CTX-M-13 around position 56. Bold letters indicate the single amino acid substitution Lys-56-Glu.
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Alignment of the amino acid sequences of <t>CTX-M-213</t> (from isolates KA1 to KA6) and CTX-M-13 around position 56. Bold letters indicate the single amino acid substitution Lys-56-Glu.
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Alignment of the amino acid sequences of <t>CTX-M-213</t> (from isolates KA1 to KA6) and CTX-M-13 around position 56. Bold letters indicate the single amino acid substitution Lys-56-Glu.
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Alignment of the amino acid sequences of <t>CTX-M-213</t> (from isolates KA1 to KA6) and CTX-M-13 around position 56. Bold letters indicate the single amino acid substitution Lys-56-Glu.
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Alignment of the amino acid sequences of <t>CTX-M-213</t> (from isolates KA1 to KA6) and CTX-M-13 around position 56. Bold letters indicate the single amino acid substitution Lys-56-Glu.
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Alignment of the amino acid sequences of <t>CTX-M-213</t> (from isolates KA1 to KA6) and CTX-M-13 around position 56. Bold letters indicate the single amino acid substitution Lys-56-Glu.
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Alignment of the amino acid sequences of <t>CTX-M-213</t> (from isolates KA1 to KA6) and CTX-M-13 around position 56. Bold letters indicate the single amino acid substitution Lys-56-Glu.
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Alignment of the amino acid sequences of <t>CTX-M-213</t> (from isolates KA1 to KA6) and CTX-M-13 around position 56. Bold letters indicate the single amino acid substitution Lys-56-Glu.
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Alignment of the amino acid sequences of <t>CTX-M-213</t> (from isolates KA1 to KA6) and CTX-M-13 around position 56. Bold letters indicate the single amino acid substitution Lys-56-Glu.
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Alignment of the amino acid sequences of CTX-M-213 (from isolates KA1 to KA6) and CTX-M-13 around position 56. Bold letters indicate the single amino acid substitution Lys-56-Glu.

Journal: Antimicrobial Agents and Chemotherapy

Article Title: First Report of an OXA-48- and CTX-M-213-Producing Kluyvera Species Clone Recovered from Patients Admitted in a University Hospital in Madrid, Spain

doi: 10.1128/AAC.01238-18

Figure Lengend Snippet: Alignment of the amino acid sequences of CTX-M-213 (from isolates KA1 to KA6) and CTX-M-13 around position 56. Bold letters indicate the single amino acid substitution Lys-56-Glu.

Article Snippet: In order to generate a plasmid encoding CTX-M-13 β-lactamase (pCTX-M-13), the recombinant plasmid pCTX-M-213 was used as a template for site-directed mutagenesis. pCTX-M-213 plasmid purification was performed by using the QIAprep spin miniprep kit high-yield protocol (Qiagen), and PCR was performed using primers CTX-M-213-K56E-F (5′-CCAAAGATAATACGCAGGTGCTTTATCGCGGTGATGAACGCTTTCC-3′) and CTX-M-213-K56E-R (5′-TATCGATGAGCGCGACGCCCAGCCGCCCTCCGCTGC-3′) (Q5 site-directed mutagenesis kit; New England BioLabs Inc.).

Techniques: